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Spodoptera frugiperda single cell suspension cell line in serum- free media, methods of producing and using
Spodoptera frugiperda single cell suspension cell line in serum- free media, methods of producing and using
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机译:无血清培养基中的草地贪夜蛾单细胞悬浮细胞系,生产和使用方法
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摘要
Disclosed and claimed is a new insect cell line, Sf900+, ATCC CRL- 12579. The insect cell line was established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 (ATCC CRL-1771) through multiple rounds of limiting dilution and selection in a serum-free insect medium supplemented with added human insulin. The insect cell line is useful in BEVS or as an adjuvant and has many characteristics and advantages. Also disclosed and claimed are recombinant proteins from recombinant baculovirus expression in insect cells such as Sf900+ cells, for instance, HA, NA, EPO, CD4, CEA, and thrombospondin.
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