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Spodoptera frugiperda single cell suspension cell line in serum- free media, methods of producing and using

机译:无血清培养基中的草地贪夜蛾单细胞悬浮细胞系,生产和使用方法

摘要

Disclosed and claimed is a new insect cell line, Sf900+, ATCC CRL- 12579. The insect cell line was established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 (ATCC CRL-1771) through multiple rounds of limiting dilution and selection in a serum-free insect medium supplemented with added human insulin. The insect cell line is useful in BEVS or as an adjuvant and has many characteristics and advantages. Also disclosed and claimed are recombinant proteins from recombinant baculovirus expression in insect cells such as Sf900+ cells, for instance, HA, NA, EPO, CD4, CEA, and thrombospondin.
机译:公开并要求保护的是新的昆虫细胞系,Sf900 +,ATCC CRL-12579。该昆虫细胞系通过多轮有限稀释和在血清中选择而从鳞翅目,夜蛾科,草地贪夜蛾Sf-9(ATCC CRL-1771)建立。补充了人类胰岛素的无昆虫培养基。昆虫细胞系可用于BEVS或作为佐剂,并具有许多特性和优点。还公开和要求保护的是来自重组杆状病毒在昆虫细胞,例如Sf900 +细胞,例如HA,NA,EPO,CD4,CEA和血小板反应蛋白中表达的重组蛋白。

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