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首页> 外国专利> New polypeptide useful as an immunoassay reagent for specific detection of antibodies to human herpes virus type 8 (HHV8) comprises part of the C-terminal sequence of the ORF 65 capsid protein of HHV8

New polypeptide useful as an immunoassay reagent for specific detection of antibodies to human herpes virus type 8 (HHV8) comprises part of the C-terminal sequence of the ORF 65 capsid protein of HHV8

机译：可用作特异性检测针对人类8型疱疹病毒（HHV8）抗体的免疫测定试剂的新多肽，包含HHV8 ORF 65衣壳蛋白C端序列的一部分

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New polypeptide (I) has an amino acid sequence belonging to the C-terminal sequence of 85 amino acids of the ORF 65 capsid protein of HHV8, or an equivalent antigenic sequence, provided that the sequence is immunoreactive with HHV8 and non-immunoreactive with Epstein-Barr virus (EBV). Independent claims are also included for the following: (1) a reagent for detecting HHV8, comprising (I); (2) a means for detecting HHV8, comprising (I) immobilized on a solid support; (3) a method for detecting anti-HHV8 antibodies in a biological sample, comprising: (a) preparing a mixture comprising the sample and a reagent (II) that is immobilized on a support as in (2) or is derived from all or part of the ORF 65 capsid protein of HHV8; (b) removing material not bound to (II) by washing; and (c) detecting any complex formed by binding between (II) and anti-HHV8 antibodies; (4) a method for detecting anti-HHV8 antibodies in a biological sample, comprising: (a) preparing a mixture comprising the sample, (II) and mono- or polyclonal antibodies that are directed against (II) and will compete with any anti-HHV8 antibodies in the sample; (b) removing material not bound to (II) by washing; and (c) detecting any complex formed by binding between (II) and anti-HHV8 antibodies that are exogenous or endogenous to the sample; (5) a method for detecting anti-HHV8 antibodies in a biological sample, comprising: (a) preparing a mixture comprising the sample, (II) and a reagent (III) derived from all or part of the ORF 73 protein of HHV8; (b) removing material not bound to the reagents by washing; and (c) detecting any complex formed by binding between the reagents and anti-HHV8 antibodies; (6) a method for detecting anti-HHV8 antibodies in a biological sample, comprising: (a) preparing a mixture comprising the sample, (II), (III) and mono- or polyclonal antibodies as in (4); (b) removing material not bound to the reagent by washing; and (c) detecting any complex formed by binding between the reagent and anti-HHV8 antibodies that are exogenous or endogenous to the sample.
机译：新多肽（I）具有的氨基酸序列属于HHV8的ORF 65衣壳蛋白的85个氨基酸的C端序列，或等同的抗原序列，只要该序列与HHV8具有免疫反应性而与Epstein不具有免疫反应性-巴尔病毒（EBV）。还包括以下方面的独立权利要求：（1）用于检测HHV8的试剂，其包含（I）; （2）检测HHV8的方法，其包括（I）固定在固体支持物上; （3）一种用于检测生物样品中抗HHV8抗体的方法，该方法包括：（a）制备包含样品和试剂（II）的混合物，该混合物固定在（2）中的载体上或衍生自全部或HHV8的ORF 65衣壳蛋白的一部分; （b）通过洗涤除去不与（II）结合的材料; （c）检测通过（II）和抗HHV8抗体之间的结合形成的任何复合物; （4）一种检测生物样品中抗HHV8抗体的方法，其包括：（a）制备包含样品，（II）和针对（II）并且将与任何抗-样品中的HHV8抗体; （b）通过洗涤除去不与（II）结合的材料; （c）检测由（II）和样品的外源性或内源性抗HHV8抗体之间的结合形成的任何复合物; （5）一种用于检测生物样品中的抗HHV8抗体的方法，其包括：（a）制备包含样品，（II）和衍生自HHV8的全部或部分ORF 73蛋白的试剂（III）的混合物; （b）通过洗涤除去不与试剂结合的物质; （c）检测通过试剂与抗HHV8抗体之间的结合形成的任何复合物; （6）一种用于检测生物样品中的抗HHV8抗体的方法，其包括：（a）制备包含样品，（II），（III）和（4）中的单克隆或多克隆抗体的混合物; （b）通过洗涤除去不与试剂结合的物质; （c）检测由试剂与样品外源或内源的抗HHV8抗体之间的结合形成的任何复合物。

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