首页>
外国专利>
RECOMBINANT BACULOVIRUS AND METHOD OF MACELISA USING THE RECOMBINANT TGEV SPIKE PROTEIN FOR THE DETECTION OF SPECIFIC ANTIBODIES TO TGEV
RECOMBINANT BACULOVIRUS AND METHOD OF MACELISA USING THE RECOMBINANT TGEV SPIKE PROTEIN FOR THE DETECTION OF SPECIFIC ANTIBODIES TO TGEV
展开▼
机译：重组杆状病毒和使用重组TGEV斑点蛋白检测TGEV特异抗体的MACELISA方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
PURPOSE: Provided are a genetic recombinant baculovirus and a recombinant spike protein of transmissible gastroenteritis virus(TGEV) manufactured therefrom. And a monoclonal antibody capture enzyme linked immunosorbent assay for detecting the antibody of TGEV using the same protein is also provided, thereby the antibody of TGEV can be rapidly detected. CONSTITUTION: The recombinant spike protein of TGEV is produced by the steps of: isolating and identifying TGEV from pigs died of diarrhea; analyzing the nucleotide sequence of spike protein of TGEV; inserting the TGEV spike gene into the baculovirus gene to produce a recombinant baculovirus(KFCC-11015); infecting the recombinant baculovirus into SF9 cells to express TGEV spike protein. The antibody of TGEV is produced by inoculating the expressed recombinant TGEV spike protein into Guinea pigs. The monoclonal antibody capture enzyme linked immunosorbent assay using TGEV spike protein that is produced from the recombinant baculovirus(KFCC-11015) in Guinea pigs as an antigen is used in detecting the neutralizing antibody of TGEV.
展开▼
