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NUCLEOTIDE SEQUENCES OF LECTINS FRACTIONATED FROM KOREAN MISTLETOE(VISCUM ALBUM COLORATUM

机译：韩国槲寄生凝集素凝集素的核苷酸序列

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摘要

PURPOSE: Lectins from Korean mistletoe are purified by chromatography and monoclonal antibodies are raised to isolate two different lectins(KML-II U and KML-II L). Gene cloning reveals that two lectins are different from lectins purified from european mistletoe. CONSTITUTION: The invention is disclosed nucleotide sequences of lectins, having molecular weight 68.8 kDa and 56.4 kDa, purified from Korean mistletoe by ammonium sulfate fractionation and Sepharose 4B column chromatography. Two PCR primers( primer 1; 5'-GATIACICATCAIACIGG, and primer 2; 5'-ACIATICGCACIGTIGGTTC) designed from part of amino sequences of purified lectins amplify about 880bp PCR product, cloned into pGEMT vector. Nucleotide sequences of two clones are determined. Nucleotide sequences show that isomers exist like lectins purified from european mistletoe. Purified lectins increase the immune response of immune system and show higher cytotoxic effect than that of lectins from european mistletoe

机译：目的：通过层析纯化来自韩国槲寄生的凝集素，并制备单克隆抗体以分离两种不同的凝集素（KML-II U和KML-II L）。基因克隆表明，两种凝集素与从欧洲槲寄生中纯化的凝集素不同。组成：本发明公开了分子量为68.8kDa和56.4kDa的凝集素的核苷酸序列，其通过硫酸铵分级分离和Sepharose 4B柱色谱法从韩国槲寄生中纯化。由纯化的凝集素的部分氨基酸序列设计的两条PCR引物（引物1; 5'-GATIACICATCAIACIGG，引物2; 5'-ACIATICGCACIGTIGGTTC）扩增约880bp的PCR产物，克隆到pGEMT载体中。确定两个克隆的核苷酸序列。核苷酸序列表明，异构体的存在类似于从欧洲槲寄生中纯化的凝集素。纯化的凝集素比欧洲槲寄生的凝集素提高免疫系统的免疫反应并显示出更高的细胞毒性作用

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公开/公告号KR20000012147A

专利类型
公开/公告日2000-02-25

原文格式PDF
申请/专利权人 KIM JONG BAE;
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申请/专利号KR19990041288
发明设计人 KIM JONG BAE;SONG SEONG GYU;SEO BYEONG SEON;LEE GWAN HEE;DO MYEONG SUL;GWAK JIN HWAN;YOO YEONG CHUN;YOON TAEK JUN;KANG TAE BONG;PARK CHUN HO;
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申请日1999-09-27
分类号C12N15/29;
国家 KR
入库时间 2022-08-22 01:46:06

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