首页> 外国专利> CLONING OF HUMAN CHOLINE/ETHANOLAMINEPHOSPHOTRANSFERASES; SYNTHESIS OF PHOSPHATIDYLCHOLINE, PHOSPHATIDYLETHANOLAMINE, AND PLATELET ACTIVATING FACTOR

CLONING OF HUMAN CHOLINE/ETHANOLAMINEPHOSPHOTRANSFERASES; SYNTHESIS OF PHOSPHATIDYLCHOLINE, PHOSPHATIDYLETHANOLAMINE, AND PLATELET ACTIVATING FACTOR

机译：人胆碱/乙醇胺磷酸转移酶的克隆;磷脂酰胆碱，磷脂酰乙醇胺和血小板活化因子的合成

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摘要

We report the first cloning and expression, from a mammalian source, of proteins capable of catalyzing choline- and ethanolaminephosphotransferase reactions (hCEPT1 and hCEPT2). Both coding regions predict highly hydrophobic proteins of 43-46.5 kDa with several predicted membrane spanning domains. A CDP-alcohol phosphotransferase motif, DG(x)2AR(x)8G(x)3D(x)3D, has been identified in both hCEPT1 and hCEPT2 choline- and ethanolamine- phosphotransferases (and several other lipid synthesizing enzymes that catalyze the formation of a phosphoester bond by the displacement of CMP from a CDP-alcohol by a second alcohol). Site-directed mutagenesis was used to differentiate the residues responsible for choline- versus ethanolamine- phosphotransferase activity. Mutation of glycine 156 of hCEPT1 abolished ethanolaminephosphotransferase activity, while cholinephosphotransferase activity remained intact.

机译：我们报告第一个克隆和表达，从哺乳动物的来源，能够催化胆碱和乙醇胺磷酸转移酶反应（hCEPT1和hCEPT2）的蛋白质。两个编码区均预测具有43-46.5 kDa的高度疏水性蛋白，并具有几个预测的跨膜结构域。在hCEPT1和hCEPT2胆碱和乙醇胺磷酸转移酶（以及其他几种催化形成脂质的脂质合成酶）中均已鉴定出CDP-醇磷酸转移酶基序DG（x）2AR（x）8G（x）3D（x）3D通过用第二种醇将CMP从CDP醇中取代而产生的磷酸酯键）。使用定点诱变来区分负责胆碱和乙醇胺磷酸转移酶活性的残基。 hCEPT1的甘氨酸156突变消除了乙醇胺磷酸转移酶的活性，而胆碱磷酸转移酶的活性保持不变。

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公开/公告号WO9964605A2

专利类型
公开/公告日1999-12-16

原文格式PDF
申请/专利权人 MCMASTER CHRISTOPHER;HENNEBERRY ANETTE;
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申请/专利号WO1999CA00513
发明设计人 MCMASTER CHRISTOPHER;HENNEBERRY ANETTE;
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申请日1999-06-07
分类号C12N15/54;C12N15/11;C12N9/12;C12Q1/68;C12P7/64;C07F9/10;C07K16/40;G01N33/50;G01N33/53;A23L1/30;
国家 WO
入库时间 2022-08-22 01:48:47

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