Vibriosis vaccine against Vibrio vulnificus produced by biotype 2 eels.

• 1. 利用WAVE波浪生物反应器生产猪圆环病毒2型灭活疫苗的方法 [P] . 中国专利： CN103520715B . 2015.05.13
• 2. 利用WAVE波浪生物反应器生产猪圆环病毒2型灭活疫苗的方法 [P] . 中国专利： CN103520715A . 2014-01-22
• 3. Vaccine against vibriosis produced by Vibrio vulnificus biotype 2 in eels. [P] . ES2127702A1 . 1999-04-16

  机译： 鳗弧菌生物型2型产生的抗弧菌病疫苗。

• 4. Recombinant recombinant vector for producing antigen for Vibrio vulnificus diagnosis and Monoclonal antibody specific to Vibrio vulnificus RtxA1 [P] . 韩国专利： KR101372766B1 . 2014-03-13

  机译： 产生用于创伤弧菌诊断的抗原的重组重组载体和针对弧菌弧菌RtxA1的单克隆抗体

• 5. Recombinant recombinant vector for producing antigen for Vibrio vulnificus diagnosis and Monoclonal antibody specific to Vibrio vulnificus RtxA1 [P] . 韩国专利： KR101349413B1 . 2014-01-10

  机译： 产生用于创伤弧菌诊断的抗原的重组重组载体和针对弧菌弧菌RtxA1的单克隆抗体

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• 5. Evidence that water transmits Vibrio vulnificus biotype 2 infections to eels. [O] . C Amaro, E G Biosca, B Fouz, 1995
• 6. Effectiveness of different vaccine formulations against vibriosis caused by Vibrio vulnificus serovar E (biotype 2) in European eels Anguilla anguilla [O] . Collado Nieto, Rosa María, Fouz Rodríguez, Belén, Sanjuán Caro, Eva, 2000
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• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. 双重PCR在创伤弧菌(Vibrio vulnificus)和副溶血弧菌(Vibrio parahaemolytious)快速检测中的应用 [J] . 张新中 ,文万侥 ,徐先栋 . 渔业信息与战略 . 2007,第009期
• 3. 双重PCR在创伤弧菌（Vibrio vulnificus）和副溶血弧菌（Vibrio parahaemolytious）快速检测中的应用 [J] . 张新中 ,文万侥 ,徐先栋 . 现代渔业信息 . 2007,第009期
• 4. 鳗源创伤弧菌的鉴定与血清型分析 [C] . 许斌福 ,XU Binfu ,龚晖 . 2016年中国南方十六省（市、区）第三十二次南方渔业论坛 . 2016
• 5. 鳗弧菌灭活疫苗研制：I鳗弧菌O1血清型灭活疫苗的中试生产和免疫效力评价II鳗弧菌三价灭活疫苗的免疫效果 [A] . 丁山 . 2018