首页> 外国专利> In vitro diagnostic procedure for the determination of dopamine - - hydroxylase activity (specific to the evaluation of the function of the sympathetic nervous system of a patient from a serum sample.And kit for the determination of in vitro activity of dopamine - hydroxylase specific B -

In vitro diagnostic procedure for the determination of dopamine - - hydroxylase activity (specific to the evaluation of the function of the sympathetic nervous system of a patient from a serum sample.And kit for the determination of in vitro activity of dopamine - hydroxylase specific B -

机译:用于测定多巴胺-羟化酶活性的体外诊断程序(特定于从血清样品中评估患者的交感神经系统的功能。以及用于测定多巴胺-羟化酶特异性B-的体外活性的试剂盒

摘要

"In vitro diagnostic procedure for the determination of dopamine beta hydroxylase specific activity for the evaluation of function of the sympathetic nervous system of a patient from a sample of SORThe, and kit for the determination of in vitro activity specific for dopamine beta hydroxylase ''.The present invention relates to a process for the determination of in vitro diagnostic activity of dopamine beta hydroxylase specific for evaluation of function of the sympathetic nervous system of a paIn a serum sample, which comprises the steps of: (a) to filter the serum sample to substantially remove drugs and endogenous inhibitors of dopamine beta hydroxylase; (b) incubating the sample filEntry of step (a) with a substrate of dopamine beta hydroxylase for conversion into a detectable compound; (c) determine the quantity of detectable compound to assess the activity of dopamine beta hydroxylASE and compare with a normal sample, by which a quantity smaller than a normal sample is indicative of a function of the sympathetic nervous system is reduced, and a quantity larger than a amostrThe normal is indicative of an increased role of the sympathetic nervous system.A reduction in the function of the sympathetic nervous system is indicative of neurodegenerative diseases, psycho affective and neuroendocrine, cardiovascular, burning, and chronic fatigue.And the effects of stress and panic syndrome. An increase in the function of the sympathetic nervous system is indicative of the effects of stress.
机译:“用于从SORThe样品中测定多巴胺β羟化酶比活性的体外诊断程序,以评估患者的交感神经系统的功能,以及测定多巴胺β羟化酶特异性的体外活性的试剂盒”本发明涉及确定多巴胺β羟化酶的体外诊断活性的方法,所述多巴胺β羟化酶对评估paIn血清样品的交感神经系统的功能具有特异性,该方法包括以下步骤:(a)过滤血清样品以基本去除多巴胺β羟化酶的药物和内源性抑制剂;(b)将步骤(a)的样品溶液与多巴胺β羟化酶的底物一起温育以转化为可检测的化合物;(c)确定要评估的可检测化合物的量多巴胺β羟化酶的活性,并与正常样品进行比较,表明该样品的量少于正常样品交感神经系统功能的降低,数量的大于正常状态表明交感​​神经系统作用的增强,交感神经系统功能的降低表明神经退行性疾病,精神情感和神经内分泌,心血管,灼热和慢性疲劳以及压力和恐慌综合症的影响。交感神经系统功能的增强表明压力的影响。

著录项

  • 公开/公告号BR9810038A

    专利类型

  • 公开/公告日2000-09-19

    原文格式PDF

  • 申请/专利权人 NEURO-BIOTECHINC.;

    申请/专利号BR19989810038

  • 发明设计人 ANDREE G. ROBERGE;MICHAEL CHAREST;

    申请日1998-05-28

  • 分类号C12Q1/26;

  • 国家 BR

  • 入库时间 2022-08-22 01:57:08

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