首页> 外国专利> ONE PAIR OF NUCLEIC ACID PROBES AND MEASUREMENT OF AMOUNT OF SPECIFIC GROUP OR SPECIFIC MICROORGANISM EXISTING IN COMPOSITE MICROORGANISM SYSTEM OR SYMBIOFIC MICROORGANISM SYSTEM WITH THE PROBES

ONE PAIR OF NUCLEIC ACID PROBES AND MEASUREMENT OF AMOUNT OF SPECIFIC GROUP OR SPECIFIC MICROORGANISM EXISTING IN COMPOSITE MICROORGANISM SYSTEM OR SYMBIOFIC MICROORGANISM SYSTEM WITH THE PROBES

机译：一对核酸问题和具有该问题的复合微生物系统或符号微生物系统中存在的特定基团或特定微生物的数量的测量

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摘要

PROBLEM TO BE SOLVED: To obtain a pair of new nucleic acid probes capable of determining the bacterial strains of a composite microorganism system by labeling plural nucleic acids capable of reacting with target base sequences, respectively, with a pair of fluorescent pigment molecules capable of causing a fluorescence resonance energy transfer phenomenon. ;SOLUTION: A pair of nucleic acid probes are obtained by labeling two oligonucleotides with a pair of fluorescent pigment molecules capable of causing a fluorescence resonance energy transfer(FRET) phenomenon. The two oligonucleotides can be hybridized with target base sequences, respectively. Therein, when the two nucleic acid probes are hybridized with the target base sequences, respectively, one of a pair of the fluorescent pigment molecules labels one of the mutually faced molecular ends of the two oligonucleotides, and the other labels the chain of the other oligonucleotide, in order that the fluorescent pigment molecules labeling the nucleic acid probes most effectively cause the fluorescence resonance energy transfer(FRET) phenomenon. A pair of the new nucleic acid probes thereby enable to simply and rapidly measure the amounts of specific bacterial stains in the composite microorganism system.;COPYRIGHT: (C)2000,JPO

机译：解决的问题：通过用分别能够与靶碱基序列反应的多个核酸标记分别能够与靶碱基序列反应的多个核酸，获得能够确定复合微生物系统的细菌菌株的一对新的核酸探针。荧光共振能量转移现象。 ;解决方案：通过用一对能够引起荧光共振能量转移（FRET）现象的荧光色素分子标记两个寡核苷酸，获得一对核酸探针。两种寡核苷酸可以分别与靶碱基序列杂交。其中，当两种核酸探针分别与靶碱基序列杂交时，一对荧光颜料分子之一标记两个寡核苷酸的彼此面对的分子末端中的一个，另一个标记另一个寡核苷酸的链。为了使标记核酸探针的荧光颜料分子最有效地引起荧光共振能量转移（FRET）现象。因此，一对新的核酸探针能够简单，快速地测量复合微生物系统中特定细菌污渍的数量。;版权所有：（C）2000，JPO

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公开/公告号JP2000184894A

专利类型
公开/公告日2000-07-04

原文格式PDF
申请/专利权人 JAPAN BIOINDUSTRY ASSOCIATION;AGENCY OF IND SCIENCE & TECHNOL;KANKYO ENG CO LTD;
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申请/专利号JP19990292861
发明设计人 KOSHO KENTA;KAMAGATA YOICHI;KOYAMA OSAMU;KURATA SHINYA;KANEKAWA TAKAHIRO;KURANE RYUICHIRO;YOKOMAKU TOYOICHI;YAMADA KAZUTAKA;
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申请日1999-10-14
分类号C12N15/09;C12Q1/04;C12Q1/68;G01N21/78;G01N33/58;
国家 JP
入库时间 2022-08-22 01:58:48

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