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PREPARATION OF HIGH CONCENTRATION AMINO ACID SOLUTION AND PEPTIDE SYNTHESIS USING HIGH CONCENTRATION AMINO ACID SOLUTION
PREPARATION OF HIGH CONCENTRATION AMINO ACID SOLUTION AND PEPTIDE SYNTHESIS USING HIGH CONCENTRATION AMINO ACID SOLUTION
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机译:用高浓度氨基酸溶液制备高浓度氨基酸溶液和肽的合成
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摘要
PROBLEM TO BE SOLVED: To provide a method for preparing high concentration amino acid solution for synthesis of dipeptides (a lower alkyl ester of aspartylphenylalanine) utilizing protease and a synthesizing method for dipeptide using the high concentration amino acid solution. ;SOLUTION: In the preparation of high concentration substrate solution used for synthesis of peptide by an enzyme reaction, a N-substituted aspartic acid and a lower alkyl ester of phenylalanine and water of the amount that includes the substrates of the concentration higher than the solubility of each substrate to water, and heating at 30°C. The reaction is carried out by adding protease to the high density solution of the amino acid obtained by the prescribed method.;COPYRIGHT: (C)2000,JPO
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