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16S RIBOSOMAL RNA GENE AND DETECTION OF KB1 STRAIN BY USING DNA SEQUENCE THIS GENE
16S RIBOSOMAL RNA GENE AND DETECTION OF KB1 STRAIN BY USING DNA SEQUENCE THIS GENE
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机译：DNA序列测定16S核糖体RNA基因并检测KB1菌株
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摘要
PROBLEM TO BE SOLVED: To obtain a new 16S rRNA gene composed of the 16S rRNA gene of Vibrio sp. KB1 strain having a specific base sequence, useful in a field of environmental clean-up or the like, and usable for the rapid, simple, specific and highly sensitive detection of this microorganism. ;SOLUTION: This new 16S rRNA gene is a new 16S rRNA gene of Vibrio sp. KB1 strain having a base sequence of the formula, and is useful as a probe, a primer or the like usable for a method or the like for detecting and counting the Vibrio sp. KB1 strain capable of degrading a hardly degradable compound such as an aromatic hydrocarbon and an organic chlorine-based compound causing soil pollution. The gene is obtained by preparing the 16S rRNA from the Vibrio sp. KB1 strain by a conventional method, constructing a DNA library by using the 16S rRNA, and screening the DNA library by using a partial fragment of the 16S rRNA gene of a known Gram-negative aerobic bacterium as a prove.;COPYRIGHT: (C)2000,JPO
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