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Thermostable firefly luciferase, thermostable firefly luciferase gene, manufacturing method of a novel recombinant DNA and heat resistance firefly luciferase,
Thermostable firefly luciferase, thermostable firefly luciferase gene, manufacturing method of a novel recombinant DNA and heat resistance firefly luciferase,
PURPOSE: To provide a new modified gene useful for the production of a heat- stable firefly luciferase. ;CONSTITUTION: A heat-stable firefly luciferase gene coding an amino acid sequence corresponding to the amino acid sequence of a wild-type firefly luciferase, luciferase of Luciola cruciata (the amino acid sequence of formula) or luciferase of Luciola lateralis provided that the 217th amino acid of the sequence is substituted with a hydrophobic amino acid (preferably isoleucine, leucine or valine). The luciferase gene is produced by site-specifically converting the 217th amino acid of the amino acid sequence of the wild-type firefly luciferase to a hydrophobic amino acid residue by treatment with reagent, irradiation with ultraviolet ray, genetic engineering technique, protein engineering technique, etc.;COPYRIGHT: (C)1993,JPO&Japio
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