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Manufacturing method null of the useful material which uses the microbe and the said microbe which possess the high manifestation vector and the said high manifestation vector
Manufacturing method null of the useful material which uses the microbe and the said microbe which possess the high manifestation vector and the said high manifestation vector
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机译：使用具有高表达载体和所述高表达载体的微生物和所述微生物的有用材料的制造方法无效
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摘要
PURPOSE:To obtain a new vector capable of highly expressing an exogenote product using Bacillus.brevis as a host microorganism. CONSTITUTION:A highly expressing vector pHY700 expressed by a restriction enzyme map of the figure. The aforementioned highly expressing vector pHY700 is obtained by treating a well-known plasmid having a DNA fragment linking an alpha-amylase (BLA) gene of Bacillus.licheniformis to the downstream side of an MWP promoter gene of Bacillus.brevis 47 (FERM P-7224) with a restriction enzyme, linking a promoter region fragment of a main extracellular protein gene (HWP gene) of Bacillus.brevis HPD31 (FERM BP-1087) thereto, transforming the Bacillus.brevis, providing a plasmid in which the MWP promoter is converted into an HWP promoter, treating the resultant plasmid with a restriction enzyme, a ligase and a linker and removing a BLA genetic region.
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