A method for increasing secondary metabolite production using clustered biosynthetic genes

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• 2. 一种利用突变OsHsfC2a基因提前水稻生育期及提高产量的方法 [P] . 中国专利： CN113512549A . 2021-10-19
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  机译： 利用簇状生物合成基因增加次生代谢产物产量的方法。

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  机译： 通过聚类生物合成基因提高次生代谢产物的方法。

• 5. INCREASING METHOD FOR PRODUCTION OF SECONDARY METABOLITES USING CLUSTERED BIOSYNTHETIC GENE [P] . 日本专利： JPH02150284A . 1990-06-08

  机译： 利用聚类生物合成基因生产次生代谢产物的方法

• 1. Fungi as chemical industries and genetic engineering for the production of biologically active secondary metabolites [J] . Abid Ali Khan, Nafees Bacha, Bashir Ahmad, 亚太热带生物医学杂志（英文版） . 2014,第011期
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• 4. Increased resistance of drought by Trichoderma harzianum fungal treatment correlates with increased secondary metabolites and proline content [J] . Mona S.Alwhibi, Abeer Hashem, Elsayed Fathi Abd_Allah, 农业科学学报（英文版） . 2017,第008期
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• 6. A Path to Biodiscovery: Isolating Antarctic Ascidian-Associated Bacteria and Screening Genomes for Secondary Metabolite-Encoded Biosynthetic Gene Clusters and Signatures of Cold Adaptation [D] . Ernster, Nathan M. 2021
• 7. Identification of Secondary Metabolite Gene Clusters in the Pseudovibrio Genus Reveals Encouraging Biosynthetic Potential toward the Production of Novel Bioactive Compounds [O] . Lynn M. Naughton, Stefano Romano, Fergal O’Gara, -1
• 8. Identification of secondary metabolite gene clusters in the Pseudovibrio genus reveals encouraging biosynthetic potential toward the production of novel bioactive compounds [O] . Naughton, Lynn M., Romano, Stefano, O'Gara, Fergal, 2017

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. OsPTR9基因的超表达能够通过增加氮肥利用效率促进植株生长和提高水稻产量 [C] . 方中明 ,杨鑫 ,夏快飞 . 2011年广东省植物学会青年学者学术研讨会 . 2011
• 3. 通过协调甘油利用和棒酸生物合成实现棒酸产量的提高 [A] . 郭德昆 . 2013