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Identification of mutant DNA sequences fast and accurate by electrospray mass spectrometry
Identification of mutant DNA sequences fast and accurate by electrospray mass spectrometry
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机译：通过电喷雾质谱快速，准确地鉴定突变DNA序列
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摘要
A method of detecting a polymorphism at a polymorphic site in the genome of an individual is described. The method involves amplifying a portion of a genomic DNA sample by polymerase chain reaction to produce an amplified segment that contains the polymorphic site, desalting the amplified segment, and determining the mass of the amplified segment by electrospray ionization mass spectrometry. Comparison of the mass of the amplified segment to a reference mass permits detection of the presence or absence of the polymorphism. Methods for heterozygosity and disease inheritance by mass spectrometry are also described. The figure shows the molecular weight transform spectrum obtained from the mass-to-charge spectrum of a 53-base oligonucleotide.
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