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Construction and expression of renilla luciferase and green fluorescent protein fusion genes
Construction and expression of renilla luciferase and green fluorescent protein fusion genes
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机译:海肾荧光素酶和绿色荧光蛋白融合基因的构建与表达
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摘要
A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.
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