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Antisense DNA constructs for expression of hybrid MRNAs driven by inducible, tissue-specific promoters

机译：用于表达由诱导型组织特异性启动子驱动的杂交MRNA的反义DNA构建体

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摘要

A gene is regulated by introducing into a cell an inducible, tissue- specific antisense DNA construct. The antisense DNA construct comprises any inducible, tissue-specific gene, into which a DNA sequence antisense to any DNA sequence of the gene targeted for regulation has been inserted. The inducible, tissue-specific antisense DNA construct transcribes a hybrid messenger RNA containing an RNA sequence antisense to a sequence of the messenger RNA of the gene targeted for regulation. The hybrid messenger RNA also contains the RNA sequence of the inducible, tissue- specific gene. Some examples of suitable inducible genes include those selected from the group consisting of mammalian cytosolic phosphoenolpyruvate carboxykinase (PEPCK) (GTP, EC 4.1.1.32), mammalian atrial natriuretic factor (ANF), and mammalian alpha myosin heavy chain (. alpha.-MHC). In a preferred embodiment, the inducible, tissue-specific gene is the rat PEPCK gene.

机译：通过将可诱导的组织特异性反义DNA构建体引入细胞来调节基因。反义DNA构建体包含任何可诱导的组织特异性基因，其中已插入了与调控靶基因的任何DNA序列反义的DNA序列。所述可诱导的组织特异性反义DNA构建体转录杂合信使RNA，所述杂信使RNA包含与用于调控的基因的信使RNA的序列反义的RNA序列。杂交信使RNA还包含可诱导的组织特异性基因的RNA序列。合适的可诱导基因的一些实例包括选自哺乳动物胞质磷酸烯醇丙酮酸羧激酶（PEPCK）（GTP，EC 4.1.1.32），哺乳动物心房利钠因子（ANF）和哺乳动物α肌球蛋白重链（α.-MHC）的基因。）。在一个优选的实施方案中，可诱导的组织特异性基因是大鼠PEPCK基因。

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公开/公告号US5858774A

专利类型
公开/公告日1999-01-12

原文格式PDF
申请/专利权人 THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK;
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申请/专利号US19950543559
发明设计人 CHRISTOPHER M. MOXHAM;CRAIG C. MALBON;
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申请日1995-10-16
分类号C12P19/34;C12N15/00;C07H21/02;C07H21/04;
国家 US
入库时间 2022-08-22 02:08:57

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