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Monitoring endospore-forming bacteria involving the amplification of DNA of conserved regions thereof
Monitoring endospore-forming bacteria involving the amplification of DNA of conserved regions thereof
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机译：监测涉及其保守区DNA扩增的内生孢子形成细菌
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摘要
Endospore-forming bacteria present in a sample are detected or identified by adding to the sample primers which are specific for conserved regions in a coding region in the genome of the bacteria, and carrying out a DNA amplification procedure. In the detection method, any of the conserved regions present in the sample are amplified, followed by analysing for the presence of the conserved regions. In the identification method, the conserved regions of the coding region are amplified, followed by analysing the amplified DNA obtained, the primers being such that they simultaneously amplify a variable coding sequence of DNA intermediate the conserved regions of the coding region, the length of the DNA fragment obtained from the amplification step being characteristic of a particular endospore-forming bacterium.
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