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Hplc - a column for the investigation of the melanin affinity of substances

机译：HPLC-研究物质黑色素亲和力的专栏

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摘要

High pressure liquid chromatography (HPLC) column for investigating the affinity of test substances (I) for melanin has a stationary phase (column filler material) consisting of silica gel to which eumelanin (EM) or phaeomelanin (PM) is covalently bonded. The EM is synthesised in vitro from L-dihydroxyphenylalanine (L-DOPA); and the PM is synthesised in vitro from L-DOPA and cysteine. The chemical bonding method also allows the use of melanins of biological origin, e.g. the melanin from the retina. For characterising the binding behaviour (corresponding to the affinity) of (I) with melanin, a defined amount of (I) is applied to the column and the corresponding retention is determined by isocratic elution. This retention depends on the amount applied and characterises the affinity of (I). The specific volume of the column is determined by applying sodium maleate having no affinity for melanin after each run.

机译：用于研究测试物质（I）对黑色素的亲和力的高压液相色谱（HPLC）色谱柱具有固定相（色谱柱填料），该固定相由硅胶组成，其中Eumelanin（EM）或Pheomelanin（PM）共价键合。 EM是由L-二羟基苯丙氨酸（L-DOPA）体外合成的。 PM是由L-DOPA和半胱氨酸在体外合成的。化学键合方法还允许使用生物来源的黑色素，例如黑色素。来自视网膜的黑色素。为了表征（I）与黑色素的结合行为（对应于亲和力），将规定量的（I）应用于色谱柱，并通过等度洗脱确定相应的保留。该保留取决于所施加的量并表征（I）的亲和力。通过在每次运行后施加对黑色素没有亲和力的马来酸钠来确定柱的比容。

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公开/公告号DE19717837C2

专利类型
公开/公告日1999-10-28

原文格式PDF
申请/专利权人 FEUERSTEIN THOMAS DR. 79102 FREIBURG DE;KNÖRLE RAINER DR. 79102 FREIBURG DE;LIMBERGER NORBERT DR. 79102 FREIBURG DE;
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申请/专利号DE1997117837
发明设计人 FEUERSTEIN THOMAS DR. 79102 FREIBURG DE;KNÖRLE RAINER DR. 79102 FREIBURG DE;LIMBERGER NORBERT DR. 79102 FREIBURG DE;
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申请日1997-04-26
分类号G01N30/26;B01D15/08;G01N30/06;G01N30/48;
国家 DE
入库时间 2022-08-22 02:13:22

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