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首页> 外国专利> A method for measuring concentrations, as well as for the continuous or discontinuous measurement of the conversion and the rate of enzymatic or not of enzymatic reactions of pyrophosphates and / or linear polyphosphates with the exclusion of cyclic polyphosphates as well as from orthophosphates in a sample as well as the application of the method

A method for measuring concentrations, as well as for the continuous or discontinuous measurement of the conversion and the rate of enzymatic or not of enzymatic reactions of pyrophosphates and / or linear polyphosphates with the exclusion of cyclic polyphosphates as well as from orthophosphates in a sample as well as the application of the method

机译：一种测量浓度的方法，以及连续或不连续测量焦磷酸盐和/或线性多磷酸盐的转化率以及酶促反应速率或酶促反应速率的方法，其中不包括环状多磷酸盐以及样品中正磷酸盐。以及该方法的应用

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摘要

The following are claimed: (A) use of the method described in the specification (see below) for detection of, and determining the concentration of, pyrophosphates, polyphosphates and other inorganic polyanions which are from natural sources or are synthetically produced; (B) use of the method described in the specification for detection of, and determining the concentration of, compounds (which are from natural sources or are synthetically produced) which have multiple negatively charged groups and which are capable of selective and high affinity binding to certain polycationic compounds; (C) use of the method described in the specification for detection of, and determining the activity of, enzymes that catalyse reactions involving: (i) pyrophosphates, polyphosphates and other inorganic polyanions which are from natural sources or are synthetically produced or (ii) compounds as described in (B); (D) use of the method described in the specification for measurement of non-enzymatic reactions of compounds described in (i) and (ii) above, and (E) use of the method described in the specification for differentiation between linear and cyclic polyphosphates. N.B. No precise definition of the method of the invention is given. Broadly, it is based on the known fact that certain metal ions can quench or enhance the fluorescence intensity of cation-sensitive fluorescence dyes. Thus, the fluorescence intensities of fura-2, fura-Red, calcein and mag-fura-red are almost totally quenched by metal ions such as Mn2+, Co2+, Ni2+, Fe2+, Cu2+, Hg2+, Tb3+, Cu+, Zn2+ and Cd2+, and the fluorescence intensities of calcium-green-1, calcium crimson and fluo-3 are enhanced by metal ions such as Cd2+, Ca2+, Hg2+, Tb3+ and Zn2+. As polyphosphates, pyrophosphates and other similar ions show a high affinity to metal ions (especially di-cations), these compete with the above dyes for binding of the metal cations. The primary effect of these cations on the dyes is thus affected by the presence of increasing concentrations of the polyphosphates, pyrophosphates or other similar ions. This effect may be used for detection and determination of these ions.
机译：要求保护以下内容：（A）使用说明书中所述的方法（见下文）检测并确定焦磷酸，聚磷酸和其他天然来源的焦磷酸，多磷酸和其他无机聚阴离子的浓度; （B）使用说明书中描述的方法检测和确定具有多个带负电荷基团并且能够选择性和高亲和力结合的化合物（来自天然来源或人工合成）某些聚阳离子化合物; （C）使用说明书中描述的方法检测和确定催化以下反应的酶的活性：（i）焦磷酸盐，多磷酸盐和其他天然来源或合成产生的无机阴离子或（ii） （B）中所述的化合物; （D）使用说明书中描述的方法测量上述（i）和（ii）中描述的化合物的非酶反应，以及（E）使用说明书中描述的方法来区分线性和环状多磷酸盐。 N.B.没有给出本发明方法的精确定义。广泛地，这是基于已知的事实，即某些金属离子可以猝灭或增强阳离子敏感性荧光染料的荧光强度。因此，fura-2，fura-Red，钙黄绿素和mag-fura-red的荧光强度几乎被金属离子如Mn 2+，Co 2+，Ni 2+，Fe < 2 +，Cu 2 +，Hg 2 +，Tb 3 +，Cu +，Zn 2+和Cd 2+以及钙绿-1的荧光强度，通过诸如Cd 2+，Ca 2+，Hg 2+，Tb 3+和Zn 2+的金属离子增强了深红色钙和fluo-3。由于多磷酸盐，焦磷酸盐和其他类似离子对金属离子（特别是二阳离子）显示出高亲和力，因此它们与上述染料竞争结合金属阳离子。这些阳离子对染料的主要作用因此受到浓度增加的多磷酸盐，焦磷酸盐或其他类似离子的存在的影响。该效应可用于检测和确定这些离子。

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