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首页> 外国专利> ATTENUATED STRAIN OF TRANSMISSIVE GASTROENTERITIS VIRUS IN PIGS (TRANSMISSIVE GASTROENTERITIS VIRUS)

ATTENUATED STRAIN OF TRANSMISSIVE GASTROENTERITIS VIRUS IN PIGS (TRANSMISSIVE GASTROENTERITIS VIRUS)

机译：猪中的传播性胃肠炎病毒的减毒菌株（传播性胃肠炎病毒）

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FIELD: veterinary science. SUBSTANCE: invention relates to preparing vaccine against swine transmissive gastroenteritis virus. New vaccine strain of swine transmissive gastroenteritis virus is obtained by attenuation of this virus by passage in culture of cells ПТП at parallel cloning and selection of clones showing antigenic differences from the parent population of virulent virus. This strain is distinguished from virulent strain of this virus by the absence of non-neutralizing antigenic determinant on the surface glycoprotein which occurs always on the corresponding glycoprotein of virulent strains of virus. This determinant is recognized by monoclonal antibodies of the clone T9C9.2 that ensures to distinct this vaccine virus from virulent strains of virus by an antigenic structure and induced seroconversion by methods of solid-phase immunoenzyme assay and histochemical immunoenzyme assay. The obtained strain is characterized by the high reproducing capability in culture cells ПТП, manifestation of typical ЦПЭ and accumulation of virus at titer up to 105 TCD 50/cm4. The strain is harmless, has no reactivity, it shows high immunogenic activity, has no contaminations with bacteria, fungi, mycoplasmas and foreign viruses. The strain shows an antigenic stability, it has no contagiousness and reversibility. The strain is deposited in collection of microorganism strains VNIIVViM at N 1941. The strain has antigenic properties ensuring to distinct its from virulent strain of virus by an antigenic structure and seroconversion induced by this vaccine strain. EFFECT: strain indicated above, improved method of preparing.
机译：领域：兽医学。本发明涉及制备抗猪传染性胃肠炎病毒的疫苗。猪传染性胃肠炎病毒的新疫苗株是通过平行克隆细胞ПТП培养并选择显示出与强毒病毒母体抗原性不同的克隆来减毒该病毒而获得的。通过在表面糖蛋白上不存在非中和性抗原决定簇来区分该菌株与该病毒的强毒株，该非中和抗原决定簇总是出现在病毒的强毒株的相应糖蛋白上。该决定簇被克隆的T9C9.2单克隆抗体所识别，该单克隆抗体可通过抗原结构和固相免疫酶测定和组织化学免疫酶测定的方法确保将该疫苗病毒与强毒株区别开来，并诱导血清转化。所获得的菌株具有在培养细胞ПТП中高繁殖力，典型的ПП表现和在滴度高达10 5 TCD 50 / cm 4 时病毒蓄积的特征。该菌株无害，无反应性，显示高免疫原性，无细菌，真菌，支原体和外来病毒的污染。该菌株显示出抗原稳定性，没有传染性和可逆性。该菌株在1941年N日保藏在微生物菌株VNIIVViM的集合中。该菌株具有抗原特性，可通过该疫苗株诱导的抗原结构和血清转化将其与病毒的强毒株区分开。效果：上述菌株，改进的制备方法。

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