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METHOD OF COUPLED ESTIMATION OF FUNCTIONAL ACTIVITY OF PERIPHERAL BLOOD NEUTROPHILS AT BRUCELLOSIS PATHOGEN EFFECT

机译:布鲁氏菌病致病作用时外周血中性粒细胞功能活性的联合估算方法

摘要

FIELD: microbiology, immunology. SUBSTANCE: analyzed blood sample is mixed with heparin and live culture of pathogen is added. Mixture is incubated at 37 C and smears are prepared in 30 and 100-120 min. Reaction results are recorded by amount of absorbed cells in the first smear, by activity of enzymes in the first and second smears and by amount of brucellae forms in the 2nd smear. Functional state is estimated by relative index of the amount of lyzed cells to the total amount of absorbed ones and by alternation of cytochemical indices in the process of opsonophagocytic reaction course. Invention can be used for estimation of immunological state of organism at brucellosis pathogen effect. EFFECT: high degree of information, guarantee of immunological state of organism estimation.
机译:领域:微生物学,免疫学。物质:分析的血液样本与肝素混合,并加入病原体的活培养物。将混合物在37℃下孵育,并在30和100-120分钟内制备涂片。通过第一涂片中吸收的细胞数量,第一和第二涂片中酶的活性以及第二涂片中布鲁氏菌形式的数量来记录反应结果。功能状态通过溶解细胞数量与吸收细胞总量的相对指数以及在调理吞噬反应过程中细胞化学指数的变化来估计。本发明可用于估计在布鲁氏菌病病原体作用下生物体的免疫状态。效果:信息量高,保证了机体估计的免疫状态。

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