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Method of improving the expression of granulocyte colony stimulating factor by fed-batch culture that controls the specific growth rate of cells
Method of improving the expression of granulocyte colony stimulating factor by fed-batch culture that controls the specific growth rate of cells
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机译:分批补料培养提高粒细胞集落刺激因子表达的方法,控制细胞的比生长速度
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摘要
The present invention relates to a method for enhancing the expression of granulocyte colony stimulating factor (G-CSF) by fed-batch culture that controls the specific growth rate of cells. More specifically, the present invention is a recombinant Escherichia coli transformed with a G-CSF expression vector using L-arabinose operon of Salmonella strain, high concentration as an algebraic feeding method (exponential feeding) to control the growth rate of the cells Incubated with L-arabinose to induce the expression of G-CSF, followed by the addition of a fed-batch medium for constant feeding fed-batch, thereby increasing the expression of recombinant G-CSF from recombinant E. coli. It is about. The method of the present invention significantly improves the cell mass and the expression level of G-CSF and improves the plasmid in Escherichia coli at the later stage of fermentation, compared to conventional culture methods such as DO-stat fed-batch method and Do-stat fed-batch method after algebraic fed-batch culture. Keep stable until
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