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Sequence of CMC-xylanase gene of Fibrobacter succinogenes S85 and its gene expression
Sequence of CMC-xylanase gene of Fibrobacter succinogenes S85 and its gene expression
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机译:琥珀酸根瘤菌S85 CMC木聚糖酶基因序列及其基因表达
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摘要
The present invention is useful for mass production of CMC-xylanase, a kind of fibrinolytic enzyme synthesized and secreted from Fibrobacter succinogenes strain, which is a fibrin degrading microorganism.;The present invention relates to a method for isolating chromosomal DNA from an anaerobic fermenting microorganism Fibrobacter succinogenes S85; Cloning the CMC-xylanase gene; Selecting an E. coli transformant having a CMC-xylanase gene and determining a DNA base sequence; And comparing the enzymatic activities of the transformants transformed with Fibrobacter succinogenes S85 and CMC-xylanase gene.
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