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Methods for Validating the Effect of Ginseng Saponin on the Expression of Antioxidant Enzyme Gene Expression and Increasing Expression of Antioxidant Enzyme Gene
Methods for Validating the Effect of Ginseng Saponin on the Expression of Antioxidant Enzyme Gene Expression and Increasing Expression of Antioxidant Enzyme Gene
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机译:验证人参皂苷对抗氧化酶基因表达和增加抗氧化酶基因表达影响的方法
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摘要
This invention examines the effect of ginseng saponin on the expression of antioxidant enzymes, superoxide dismutase 1 (SOD1) and catalase, and confirms the useful ginseng saponin components and increases the gene expression of antioxidant enzymes To be useful for. Ginsenoside components affecting the increased gene expression of SOD1 and catalase were identified as diol-based saponins, in particular ginsenosides-Rb1 and-Rb2. The expression of the antioxidant enzyme gene is cloned into the structural gene of chloramphenicol acetyltransferase (CAT) and introduced into animal cells after the transcriptional regulatory region of the antioxidant enzyme gene is cloned. Then, the fraction of ginseng saponin is treated to express SOD1 and the catalase gene And the fractions of ginseng saponin that induce the increase. According to the present invention, it is confirmed that the antioxidant enzyme gene, which plays a key role in converting harmful oxygen harmful to human body into water and oxygen, is increased by diol-based saponin, so that the saponin is easily separated from the ginseng and is obtained in a relatively large amount Rb1 /RTI and-Rb2 can be selectively used for increasing the expression of the antioxidant enzyme gene.
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