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MEDIUM FOR LONG-TERM PROLIFERATION AND DEVELOPMENT OF CELLS

机译:长期增殖和发展细胞的媒介

摘要

A serum-free or serum-depleted medium for the short- and long-term proliferation and development of cells, particularly hematopoietic cells and bone marrow stromal cells, the medium comprising cell proliferation and development effective amounts of: a standard culture medium such as Iscove's modified Dulbecco's medium; serum albumin; transferrin; a source of lipids and fatty acids; cholesterol; a reducing agent; pyruvate; a glucocorticoid (when the cells to be cultured are hematopoietic cells); nucleosides for synthesis of DNA and RNA; growth factors that stimulate the proliferation and development of stromal cells and cells from a variety of tissues or organs, such as epidermal growth factor, fibroblast growth factor, platelet derived growth factor, and insulin; and extracellular matrix materials, such as collagen, fibronectin, and laminin.
机译:用于细胞,特别是造血细胞和骨髓基质细胞短期和长期增殖和发育的无血清或贫血清培养基,该培养基包含细胞增殖和发育的有效量的标准培养基,例如Iscove's改良的Dulbecco培养基;血清白蛋白转铁蛋白脂质和脂肪酸的来源;胆固醇;还原剂;丙酮酸糖皮质激素(当要培养的细胞是造血细胞时);用于合成DNA和RNA的核苷;刺激基质细胞和来自各种组织或器官的细胞增殖和发育的生长因子,例如表皮生长因子,成纤维细胞生长因子,血小板衍生生长因子和胰岛素;和细胞外基质材料,例如胶原蛋白,纤连蛋白和层粘连蛋白。

著录项

  • 公开/公告号EP0703978B1

    专利类型

  • 公开/公告日1999-08-18

    原文格式PDF

  • 申请/专利权人 AMGEN INC;

    申请/专利号EP19940920264

  • 发明设计人 PONTING IAN L.O.;

    申请日1994-06-17

  • 分类号C12N5/06;C12N5/08;

  • 国家 EP

  • 入库时间 2022-08-22 02:20:10

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