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Expression cassette comprising a promoter derived from gene pho4 of Schizosaccharomyces pombe

机译:包含源自粟酒裂殖酵母基因pho4的启动子的表达盒

摘要

The invention concerns the combined use of two expression cassettes, one of which is for expressing a useful DNA fragment controlled by a thiamine-regulable promotor region, while the other is for expressing an activator gene, and the host cells used for carrying this invention. The invention also concerns an expression cassette, wherein the useful ADN fragment is controlled by a promoter region derived from the pho-4 Schizosaccharomyces pombe gene as well as the vectors and cells comprising such an expression cassette. A novel method for the production of a useful protein is also provided.
机译:本发明涉及两种表达盒的组合用途,其中一种用于表达由硫胺素可调节的启动子区域控制的有用的DNA片段,而另一种用于表达激活基因,以及用于实施本发明的宿主细胞。本发明还涉及一种表达盒,其中有用的ADN片段由衍生自pho-4裂殖酵母属pombe基因的启动子区域以及包含这种表达盒的载体和细胞控制。还提供了生产有用蛋白的新方法。

著录项

  • 公开/公告号EP0749489B1

    专利类型

  • 公开/公告日1998-12-16

    原文格式PDF

  • 申请/专利权人 TRANSGENE S.A.;

    申请/专利号EP19950911383

  • 申请日1995-03-09

  • 分类号C12N15/81;C12N1/19;

  • 国家 EP

  • 入库时间 2022-08-22 02:21:10

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