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NUCLEIC ACID STAIN, DETECTION OF DOUBLE-STRANDED NUCLEIC ACID USING THE SAME AND DETECTION REAGENT FOR TARGETED NUCLEIC ACID
NUCLEIC ACID STAIN, DETECTION OF DOUBLE-STRANDED NUCLEIC ACID USING THE SAME AND DETECTION REAGENT FOR TARGETED NUCLEIC ACID
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机译：核酸污渍，使用相同的双链核酸检测和目标核酸检测试剂
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摘要
PROBLEM TO BE SOLVED: To obtain a nucleic acid stain useful for gene diagnosis by including a specific hydroxynaphthylazohydroxynaphthalenesulfonic acid salt (derivative) so as to interact with a double-stranded nucleic acid to effect change of fluorescence intensity and fluorescence wavelength and improve assay accuracy. ;SOLUTION: This nucleic acid stain comprises a 4-(2-hydroxy-1-naphthylazo)-3- hydroxy-1-naphthalenesulfonic acid salt (derivative) of the formula (R1 is OH, an alkyl, phenyl, amino or anilino; R2 is SO3Na, SO3K or SO3Li). It is preferable that a double-stranded nucleic acid is detected by measuring the change of the fluorescence properties caused by the interaction of this compound with the double-stranded nucleic acid, and it is also preferable that the other objective detection reagent for a targeted nucleic acid is prepared by including the compound of the formula and a probe consisting of a single-stranded nucleotide having a nucleic acid sequence complementary to the targeted nucleic acid.;COPYRIGHT: (C)1998,JPO
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机译：解决的问题：通过包含特定的羟基萘基偶氮羟基萘磺酸盐（衍生物），从而与双链核酸相互作用，以实现荧光强度和荧光波长的变化并提高测定准确性，从而获得可用于基因诊断的核酸染色剂。 ;解决方案：该核酸染色剂包含式（R 1 为OH，下同）的4-（2-羟基-1-萘偶氮）-3-羟基-1-萘磺酸盐（衍生物）烷基，苯基，氨基或苯胺基; R 2 是SO 3 Na，SO 3 K或SO 3 Li ）。优选通过测量由该化合物与双链核酸的相互作用引起的荧光特性的变化来检测双链核酸，并且还优选用于靶核酸的另一种目标检测试剂。通过包括下式的化合物和由具有与靶核酸互补的核酸序列的单链核苷酸组成的探针制备酸：COPYRIGHT：（C）1998，JPO
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