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Method for amplifying nucleic acid sequences by strand displacement using DNA/RNA chimeric primers
Method for amplifying nucleic acid sequences by strand displacement using DNA/RNA chimeric primers
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机译：使用DNA / RNA嵌合引物通过链置换来扩增核酸序列的方法
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摘要
A target nucleic acid may be amplified in the presence of an enzymatic system including DNA polymerase, strand translocation and RNAse H activities by using a chimeric primer that includes, in the 5' to 3' direction, an RNA-type segment capable of hybridizing with a 3'- terminal segment of the target and a DNA-type segment capable of hybridizing with a segment adjacent to the 3'-terminal segment of the target and a DNA- or RNA-type primer capable of hybridizing with the 3'- terminal segment of the target. A cyclic amplification that may be implemented isothermally on the basis of either a DNA or an RNA target is achieved even when the terminals are not defined.
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