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Synthetic peptides and process of using same for the detection of antibodies to human immunodeficiency virus (HIV) gp120 envelope protein, diagnosis of AIDS and pre-AIDS conditions and as vaccines
Synthetic peptides and process of using same for the detection of antibodies to human immunodeficiency virus (HIV) gp120 envelope protein, diagnosis of AIDS and pre-AIDS conditions and as vaccines
This invention relates to a method using synthetic peptides as the solid phase immunoadsorbent for the detection and elicitation of antibodies to Human Immunodeficiency Virus (HIV) gp120, and, in particular, antibodies having HIV neutralizing capabilities. The amino acid sequences of the peptides correspond to segments of the external envelope protein gp120 of HIV. These peptides have been found to be highly immunogenic, and are reactive with antibodies in sera of patients with AIDS, ARC or HIV infected individuals. They can also be used to elicit the production of neutralizing antibodies to HIV. More specifically, the present invention is directed to the use of a synthetic peptide selected from the groups consisting of peptides containing thirty- three amino acids in a prescribed sequence derived from the HIV-gp120 external protein, analogues, mixtures and poly-L- lysine polymers thereof, for the detection and elicitation of antibodies to HIV-gp120. It is particularly useful for the detection and elicitation of antibodies having HIV neutralizing capabilities. The detection method includes an enzyme linked immunoassay and other forms of immunoassay procedures. The present invention also relates to a method for generating high titer neutralizing antibodies to HIV gp120 protein in healthy mammals, including humans, by the use of the synthetic peptides, their analogues or mixtures in either a conjugated or a polymeric form as a key component in a synthetic vaccine for the prevention of AIDS.
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