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Molecular cloning and characterization of the feline immunodeficiency virus isolate PPR
Molecular cloning and characterization of the feline immunodeficiency virus isolate PPR
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机译：猫免疫缺陷病毒分离株PPR的分子克隆和鉴定
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摘要
Molecular clones of the feline immunodeficiency virus isolate PPR (FIV. sub.PPR) were obtained from a genomic library prepared from infected feline peripheral blood lymphocytes (PBLs). FIV.sub.PPR infected and replicated efficiently in feline PBLs but not Crandall feline kidney (CRFK) or G355-5 cells. In contrast, a clone designated 34TF10 of the prototypical FIV Petaluma isolate (FIV.sub.Pet) replicated inefficiently on feline PBLs while readily infecting and replicating in CRFK and G355-5 cells. The 34TF10 and PPR clones have an overall nucleic acid sequence identity of 91% while the env genes display only 85% conservation at the amino acid level. The long terminal repeats (LTRs) were 7% divergent between the two clones, with a lack of conservation in putative NF-&kgr; B, LBP-1, and CCAAT enhancer promoter sites. Full-length proviral clones will provide important biochemic, immunologic, and diagnostic reagents.
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