首页> 外国专利> Cloning and expression vectors in an actinomycetes strain, process for transformation of this strain, actinomycetes strain obtained and preparation of proteins

Cloning and expression vectors in an actinomycetes strain, process for transformation of this strain, actinomycetes strain obtained and preparation of proteins

机译:放线菌菌株的克隆和表达载体,该菌株的转化方法,获得的放线菌菌株和蛋白质的制备

摘要

The present invention relates to a vector for cloning and expression of a DNA sequence coding for a particular protein in an Actynomycetes strain. The process comprises:PPan attachment sequence att,PPa DNA sequence coding for an int sequnce functional in said strain, andPPa DNA sequence coding for said specific protein and for the elements ensuring the expression of said sequence in said strain.
机译:本发明涉及一种用于在放线菌菌株中克隆和表达编码特定蛋白质的DNA序列的载体。该方法包括:-附着序列att,-编码在所述菌株中有功能的int序列的DNA序列,和-p-编码在所述特定蛋白中的DNA序列。用于确保所述序列在所述菌株中表达的元件。

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