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Human manganese superoxide dismutase cDNA, its expression in bacteria and methods for the purification of enzymatic active human manganese superoxide dismutase and pharmaceuticals
Human manganese superoxide dismutase cDNA, its expression in bacteria and methods for the purification of enzymatic active human manganese superoxide dismutase and pharmaceuticals
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机译：人锰超氧化物歧化酶cDNA，其在细菌中的表达以及酶促活性人锰超氧化物歧化酶的纯化方法和药物
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摘要
A double-stranded cDNA molecule which includes DNA encoding human manganese super oxide dismutase has been created. The sequence of one strand of a double- stranded DNA molecule which encodes human manganese superoxide dismutase has been discovered. Such mole- cules may be introduced in procaryotic, e.g., bacteri- al, or eukaryotic, e.g. , yeast or mammalian, cells and the resulting cells cultured or grown under suitable conditions sa as to produce human manganese superoxide dismutase or analogs thereof which may then be recov- ered. Human MnSOD or analogs thereof may be used to catalyze the reduction of superoxide radicals, reduce reperfusion injury, prolong the survival time of iso- lated organs, or treat inflammations. The invention also concerns a method of producing enzy- matically active human manganese superoxide dismutase or an analog thereof in a bacterial cell which contains and is capable of expressing a DNA sequence encoding the superoxide dismutase by maintaining the bacterial cell under suitable conditions and in a suitable pro- duction medium. The production medium is supplemented with an amount of Mn++ so that the concentration of Mn++ in the medium is greater than about 2 ppm. This invention also concerns a method of recovering purified enzymatically active manganese super oxide dismutase from bacterial cells.
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