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Detection of nucleic acids using G-quartets (Detection of nucleic acids using G-quartets)
Detection of nucleic acids using G-quartets (Detection of nucleic acids using G-quartets)
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机译:使用G四重奏检测核酸(使用G四重奏检测核酸)
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摘要
The present invention relates to oligonucleotides that form a G-quartet structure that has been found useful in fluorescence assays to detect selected nucleic acid sequences. When one end of the oligonucleotide is labeled with a donor fluorophore and the other end is labeled with a receptor dye, the molecule, which is a G-quartet structure, is folded so that the donor-acceptor pair comes very close, The reaction between the two labels results in a loss of fluorescence of the donor or a change in other fluorescence as a result of the close proximity of the two dyes. The G-quatet structure becomes unfolded when hybridized to its complementary sequence, increasing the distance between the two dye labels. This causes a decrease in the emission of the donor or a change in the fluorescence parameter associated with other proximity. An increase in donor fluorescence intensity or a change in other fluorescence parameters can be observed as an indicator of the presence of the selected nucleic acid sequence. Alternatively, in some cases a decrease in receptor fluorescence as an indicator of the presence of the selected nucleic acid sequence may be observed if the receptor is also fluorescent.
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