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METHOD FOR IDENTIFYING ATTENUATED CHICKENPOX VIRUS OKA STRAIN OR STRAIN ORIGINATING THEREIN AND ACCEPTABLE AS ATTENUATED CHICKENPOX VACCINE VIRUS
METHOD FOR IDENTIFYING ATTENUATED CHICKENPOX VIRUS OKA STRAIN OR STRAIN ORIGINATING THEREIN AND ACCEPTABLE AS ATTENUATED CHICKENPOX VACCINE VIRUS
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机译：识别减毒的鸡痘病毒的方法冈田株或源自其的菌株以及可接受的减毒的鸡痘疫苗病毒
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摘要
Disclosed is a method for exact identification of the attenuated varicella virus Oka strain or a strain derived therefrom capable of functioning as an attenuated varicella live vaccine virus, which comprises analyzing the difference in the genomic DNA and fragments thereof between the Oka strain and a sample varicella strain, and determining whether or not a sample strain satisfies all of the following eight characteristics: the sizes of the HpaI-K fragment and the EcoRI-P fragment; the size of R2-487 region of Gene14 and the analysis by PCR-SSCP; the sizes of the restriction fragments obtained by digesting the R2-1764 fragment with AccIII; the absence or presence of a PstI cleavage site; the homology of the amino acid sequences coded by R2-487 coding region; and the homology of the amino acid sequences coded by the coding region of VZV Gene14. Also disclosed are an isolated virus strain which is substantially the same virus strain as identified by the above method; an attenuated varicella virus live vaccine comprising the same virus strain as identified by the above method; and an attenuated varicella virus Oka strain antigen.
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