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METHOD FOR DETECTING NONSENSE MUTATIONS AND FRAMESHIFT MUTATIONS
METHOD FOR DETECTING NONSENSE MUTATIONS AND FRAMESHIFT MUTATIONS
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机译：检测无意义变异和框架变异的方法
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摘要
A method for conveniently detecting nonsense mutations and frameshiftmutations, which is applicable even to large-sized test DNAs. This methodcomprises inserting, into a vector containing a promoter, an initiation codonlocated downstream the promoter and a reporter gene located downstream theinitiation codon which is a structural gene under the regulation by thepromoter and a fused polypeptide consisting of the polypeptide encoded bywhich and another polypeptide bonded to the N-terminus of the formerpolypeptide can be detected based on the function of the polypeptide encodedby the structural gene, a test nucleic acid fragment which makes the readingframe of the reporter gene to match the above-mentioned initiation codon whenit is a normal type one to a site downstream the initiation codon but upstreamthe reporter gene; expressing the test nucleic acid fragment in therecombinant vector thus obtained and the reporter gene downstream the same inhost cells; and thus examining whether or not a fused polypeptide having thefunction of the polypeptide encoded by the reporter gene is produced.
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