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AN ISOLATED DNA MOLECULE ENCODING A TOXIN PROTEIN OF THE CRY1E TYPE WHICH IS ACTIVE AGAINST HELIOTHIS/ITAL BUT NOT SPODOPTERA EXIGUA; ENTOMOCIDAL COMPOSITIONS AGAINST LEPIDOPTERAN PESTS, TRANSGENIC PLANTS
AN ISOLATED DNA MOLECULE ENCODING A TOXIN PROTEIN OF THE CRY1E TYPE WHICH IS ACTIVE AGAINST HELIOTHIS/ITAL BUT NOT SPODOPTERA EXIGUA; ENTOMOCIDAL COMPOSITIONS AGAINST LEPIDOPTERAN PESTS, TRANSGENIC PLANTS
The present invention is drawn to a novel toxin gene purified and isolated from Bacillus thuringiensis var kurstaki and given the designation CryIE(c). The novel toxin gene encodes a protein of approximately 130 kDa in size and is active against Lepidopteran insects. Also included in the invention are the proteins encoded by CryIE(c). Further disclosed are recombinant genes and proteins derived from CryIE(c). Also provided are biologically pure bacterial strains transformed with the CryIE(c) gene which can be used in entomocidal formulations for the control of Lepidopteran insects. Yet another aspect of the invention are plants transformed with the toxin gene or active fragments thereof, particularly where the transforming sequences have been optimized for expression in maize.
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