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Method for in situ or ex situ bioremediation of hexavalent chromium contaminated soils and/or groundwater

机译:六价铬污染土壤和/或地下水的原位或异位生物修复方法

摘要

A method of reducing the concentration of Cr(VI) in a liquid aqueous residue comprises the steps of providing anaerobic Cr(VI) reducing bacteria, mixing the liquid aqueous residue with a nutrient medium to form a mixture, and contacting the mixture with the anaerobic Cr(VI) reducing bacteria such that Cr(VI) is reduced to Cr(III). The anaerobic Cr(VI) reducing bacteria appear to be ubiquitous in soil and can be selected by collecting a soil sample, diluting the soil sample with a sterile diluent to form a diluted sample, mixing the diluted sample with an effective amount of a nutrient medium and an effective amount of Cr(VI) to form a mixture, and incubating the mixture in the substantial absence of oxygen such that growth of Cr(VI) sensitive microorganisms is inhibited and growth of the anaerobic Cr(VI) reducing bacteria is stimulated. A method of in situ bioremediation of Cr(VI) contaminated soil and/or groundwater is also disclosed.
机译:降低液态含水残余物中的Cr(VI)浓度的方法包括以下步骤:提供厌氧的Cr(VI)还原细菌,将液态含水残余物与营养培养基混合以形成混合物,并使混合物与厌氧性接触Cr(VI)还原细菌,使得Cr(VI)还原为Cr(III)。减少厌氧性Cr(VI)的细菌似乎在土壤中普遍存在,可以通过收集土壤样品,用无菌稀释剂稀释土壤样品以形成稀释样品,将稀释的样品与有效量的营养培养基混合来进行选择。有效量的Cr(VI)形成混合物,并在基本不存在氧气的情况下孵育混合物,从而抑制Cr(VI)敏感微生物的生长并刺激厌氧性Cr(VI)还原菌的生长。还公开了一种对Cr(VI)污染的土壤和/或地下水进行原位生物修复的方法。

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