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Method for using lead-sensitive enzymes to determine levels of lead in blood and other types of samples

机译:使用铅敏感酶测定血液和其他类型样品中铅含量的方法

摘要

A method is described for determining the level of lead in blood and other types of samples. The level of lead is determined by using a lead- sensitive enzyme. As a first step, a native lead-sensitive enzyme in the sample is disabled. This step may be omitted if the sample is known not to contain any lead-sensitive enzymes. A quantity of lead- sensitive enzyme with known enzymatic activity is then added to the sample. The new enzymatic activity of the sample is then measured. Finally, the level of lead in the sample is determined from the measurement of the new enzymatic activity, which differs from the known enzymatic activity if lead is present in the sample.
机译:描述了一种用于确定血液和其他类型样品中铅含量的方法。铅水平通过使用铅敏感酶来确定。第一步,禁用样品中的天然铅敏感酶。如果已知样品不包含任何铅敏感酶,则可以省略此步骤。然后将一定数量的具有已知酶活性的铅敏感酶添加到样品中。然后测量样品的新酶活性。最后,样品中铅的含量由新酶活性的测定确定,如果样品中存在铅,则该酶活性与已知酶活性不同。

著录项

  • 公开/公告号US5679538A

    专利类型

  • 公开/公告日1997-10-21

    原文格式PDF

  • 申请/专利权人 DAVIDOW;BERNARD;

    申请/专利号US19940200052

  • 发明设计人 BERNARD DAVIDOW;

    申请日1994-02-22

  • 分类号C12Q1/34;G01N33/20;

  • 国家 US

  • 入库时间 2022-08-22 03:09:14

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