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METHOD OF PRORENNIN PREPARING, METHOD OF PREPARING YEAST STRAIN - A PRODUCER OF PRORENNIN, METHOD OF PREPARING RECOMBINANT PLASMID DNA PX-33, METHOD OF PREPARING RECOMBINANT PLASMIN DNA PX-22, METHOD OF PREPARING RECOMBINANT PLASMID DNA PX-11
METHOD OF PRORENNIN PREPARING, METHOD OF PREPARING YEAST STRAIN - A PRODUCER OF PRORENNIN, METHOD OF PREPARING RECOMBINANT PLASMID DNA PX-33, METHOD OF PREPARING RECOMBINANT PLASMIN DNA PX-22, METHOD OF PREPARING RECOMBINANT PLASMID DNA PX-11
FIELD: biotechnology, molecular biology, genetic engineering. SUBSTANCE: prorennin is prepared by construction of recombinant plasmid DNA PLS/3 or PXX-11, or PX-22, or PX-33, or PX-34, transformation of the strains L. lipolytica with prepared recombinant DNAs followed by transformants cultivation, isolation and purification of the end product. Strains L. lipolytica ATCC - 206.88, or 20774, or 20794 were isolated. Construction of plasmids PXX-33, PXX-22 and PXX-11 is described. EFFECT: improved method of preparing. 2 cl, 17 dwg
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