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Process for the preparation of acarbiosyl transferases and methods of use for the conversion of acarbose homologues to acarbose and for the preparation of acarbose homologues

机译：用于制备阿卡波糖基转移酶的方法以及用于将阿卡波糖同系物转化为阿卡波糖和用于制备阿卡波糖同系物的方法

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摘要

The present invention relates to acarbiosyl transferases (ATs) from actinomycetes, mainly Actinoplanes sp. SE 50/13 or SE 50/110 and variants thereof, isolation, purification and characterization of this enzyme. Methods, isolation and characterization of acbD gene encoding acarbiosyl transferase, expression of acarbiosyl transferase in heterologous host organisms, conversion of acarbose minor components to acarbose and acarbose phase Use of acarbiosyl transferase for the preparation of fuselage, use in acarbose tablets of acarbiosyl transferase, and production variants in which formation of minor components is reduced by inactivation of acarbiosyl transferase. It is about manufacture.;Acarbose is effectively substituted by the maltose moiety of the acarbose like minor component formed during fermentation using an acarbioosyl transferase having the ability to substitute the maltosyl residue of the acarbose of the present invention. Can be formed.

机译：本发明涉及来自放线菌，主要是放线菌属（Actinoplanes sp。）的碳酰氨基转移酶（AT）。 SE 50/13或SE 50/110及其变体，该酶的分离，纯化和表征。方法，分离和表征编码acarbiosyl转移酶的acbD基因，异源宿主生物中acarbiosyl转移酶的表达，acarbose微量成分转化为acarbose和acarbose相的使用acarbiosyl转移酶用于制备机身，在acarbose片剂中使用acarbiosyl转移酶和生产变型，其中微量成分通过无糖基转移酶的失活而减少。这是关于制造的。阿卡波糖被具有能力取代本发明的阿卡波糖的麦芽糖残基的能力的阿卡波糖基转移酶有效地替换为在发酵过程中形成的阿卡波糖的麦芽糖部分样次要成分。可以形成。

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公开/公告号KR970065709A

专利类型
公开/公告日1997-10-13

原文格式PDF
申请/专利权人 볼프강 페트로빅키 만프레트 조벨;
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申请/专利号KR19970009945
发明设计人 크루에거 안넬리제;델베크 한스-게오르그;렌즈 위르겐 게오르그;쉬뢰더 베르너;파페 헤르만;고에케 클라우스;샤퍼 베아테;헴커 미카엘;피에퍼스베르크 볼프강;디스틀러 위르겐;스트라트만 안스가르;
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申请日1997-03-22
分类号C12N9/10;
国家 KR
入库时间 2022-08-22 03:16:19

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