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Development of Virus-Resistant Plants Using Dominant Sequence of Domains in the Replication Enzyme of Tobacco Mosaic Virus
Development of Virus-Resistant Plants Using Dominant Sequence of Domains in the Replication Enzyme of Tobacco Mosaic Virus
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机译：利用烟草花叶病毒复制酶中主要结构域的序列开发抗病毒植物
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摘要
The present invention relates to a method for developing a virus-resistant plant by introducing a domain 1 sequence existing in the replication enzyme of a tobacco mosaic virus into a plant, and a plant having a new trait of virus resistance developed by the method.;A domain 1 base sequence is cut out from a complementary DNA (CDNA) containing a whole gene of a tobacco mosaic virus and inserted into an expression vector of a plant to construct a plasmid. Then, the domain 1 sequence is introduced into the plant by the co-cultivation method of Agrobacterium . Genetic analysis confirmed the introduction and expression of the domain 1 sequence in the transgenic plants. As a result of the investigation of the degree of virus resistance against the transgenic plants, all of the transgenic plants into which the domain 1 sequence was introduced showed complete resistance to viruses, and at very high virus vaccination concentration (100 g / ml) and tobacco mosaic virus (TMV RNA, 10 g / ml). The presence of the domain 1 sequence in the transgenic tobacco plants completely inhibits the systemic disease development and vigilance of the virus associated with tobacco mosaic virus infection. Even in the case of plant RNE viruses which have been confirmed only for the tobacco mosaic virus of the present invention but are not related to each other, namely, cucumber mosaic virus, alfalfa mosaic virus, fotex virus, bromovirus, 1, the present invention is applied to all viruses having a domain 1 motif on their genome, and since motifs similar to domain 1 are also present in the RANE virus of an animal The same effect will be obtained and the present invention is applied.
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机译：本发明涉及通过将烟草花叶病毒的复制酶中存在的结构域1序列引入植物中来开发抗病毒植物的方法，以及通过该方法开发的具有新的抗病毒性的植物。从包含烟草花叶病毒的完整基因的互补DNA（CDNA）切出结构域1的碱基序列，并插入植物的表达载体中以构建质粒。然后，通过农杆菌的共培养方法将结构域1序列引入植物中。遗传分析证实了结构域1序列在转基因植物中的引入和表达。研究病毒对转基因植物的抗性程度后，引入了结构域1序列的所有转基因植物均显示出对病毒的完全抗性，并且在很高的疫苗接种浓度下（100 g / ml）和烟草花叶病毒（TMV RNA，10微克/毫升）。转基因烟草植物中域1序列的存在完全抑制了与烟草花叶病毒感染有关的病毒的全身性疾病发展和警惕性。即使在仅针对本发明的烟草花叶病毒已经证实但彼此不相关的植物RNE病毒的情况下，即黄瓜花叶病毒，苜蓿花叶病毒，fotex病毒，溴病毒1，本发明术语“结构域”应用于所有在其基因组上具有结构域1的基序的病毒，并且由于在动物的RANE病毒中也存在与结构域1相似的基序，因此将获得相同的效果，并且可以应用本发明。
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