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Process for the biobleaching of eucalyptus kraft pulp with xylanases from Aspergillus niger and isolation of this strain

机译:用黑曲霉木聚糖酶对桉树牛皮纸浆进行生物漂白的方法及该菌株的分离

摘要

This invention relates to the process of isolating the strain of Aspergillus niger par usamii, designated as Aspergillus niger CCMI 850. Identification of the strain was confirmed using microbiological methods and electron scanning microscopy. The scope of the invention also includes the process of fermenting the said strain at a temperature of 30 degree C and a pH of 5.0. This new strain can utilize an extensive range of substrates, including complex organic sources of carbon and/or nitrogen, as well as clearly-specified chemically synthesized means to produce high concentrations of xylanolytic enzymes. More specifically, this new strain produces the highest quantity of xylanase ever found in a strain of A. niger. Xylanolytic enzymes from the said xylanolytic product which accumulate outside the cells in the fermentation broth can be applied directly under specified conditions to the biobleaching of kraft pulp. Its application results in a fall in the kappa index and simultaneously an increase in the gloss of the paper obtained using these biocatalysts. IMAGE
机译:本发明涉及分离黑曲霉菌株的方法,命名为黑曲霉CCMI850。使用微生物学方法和电子扫描显微镜确认了该菌株的鉴定。本发明的范围还包括在30℃的温度和5.0的pH下发酵所述菌株的方法。这种新菌株可以利用广泛的底物,包括碳和/或氮的复杂有机源,以及明确指定的化学合成方法来生产高浓度的木聚糖分解酶。更具体地说,该新菌株产生了在黑曲霉菌株中发现的最高数量的木聚糖酶。来自所述木聚糖分解产物的木聚糖分解酶在发酵液的细胞外积累,可以在特定条件下直接应用于牛皮纸浆的生物漂白。它的应用导致κ指数下降,同时使用这些生物催化剂获得的纸的光泽度增加。 <图像>

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