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The roller bottle for simultaneous culture of the cell which minds the film and simultaneous culture manner null of the cell which uses that
The roller bottle for simultaneous culture of the cell which minds the film and simultaneous culture manner null of the cell which uses that
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机译:注意膜的细胞同时培养的滚瓶和使用该方法的细胞同时培养方式无效
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摘要
A roller bottle for trans-membrane co-culture of cells includes an exterior receptacle with a longitudinal axis having a first chamber surrounded by a sidewall. The exterior receptacle has a first neck at one end having an opening therethrough providing fluid access to said first chamber. The roller bottle further includes an interior container with a longitudinal axis and a second chamber, the interior container being located coaxially within the exterior receptacle. The interior container has a second neck at one end providing fluid access to the second chamber. The exterior receptacle is formed from a material that is substantially impermeable to gas and liquid and is sealed in a substantially fluid tight fashion forming the first chamber that has fluid access by the first neck. At least a portion of the interior container is formed from a microporous material. The roller bottle of the invention enables culturing one cell type in the presence of another cell type for the study of interactions between the cells while maintaining a physical separation between the cell types providing a scale up in cell population size from well type inserts. The microporous membrane allows free interchange of media soluble cellular products between the cell types, but maintains physical separation. MATH
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