首页>
外国专利>
Selective detection of viable and infectious cryptosporidium oocysts with the help of the polymerase chain reaction (PCR)
Selective detection of viable and infectious cryptosporidium oocysts with the help of the polymerase chain reaction (PCR)
展开▼
机译：借助聚合酶链反应（PCR）选择性检测存活和感染性隐孢子虫卵囊
展开▼
页面导航
摘要
著录项
相似文献
摘要
With the help of PCR it is possible to detect parasites of the genus Cryptosporidium by amplifying specific gene sequences. The disadvantage of this method is that it does not allow a statement about whether the detected gene sequences have been derived from viable and infectious parasites or from dead organisms or even from free DNA which has been released from organisms which have already disintegrated. However, by the combination of PCR with other known methods, i.e. an in vitro excystation assay and the removing or the destruction of nucleic acids which might be present in a sample material outside of Cryptosporidium oocysts before excystation this problem can be solved. Due to this combination the result of a PCR for Cryptosporidium allows a judgement on the infectivity of the examined material which would not be possible without this combination. Thus the fundamental advantages of PCR (high sensitivity and specificity, objective results, possibility of processing several samples in parallel) become available for applications in the field of environmental and food hygiene where it is essential to be able to answer the question whether a sample material is infectious or not.
展开▼
