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Callus cell induction from partially submerged explant tissue in liquid medium for preparation of taxanes
Callus cell induction from partially submerged explant tissue in liquid medium for preparation of taxanes
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机译：在液体培养基中从部分浸没的外植体组织中诱导愈伤组织细胞来制备紫杉烷
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摘要
A method for the induction of callus cells capable of producing taxanes from explant tissue by partially submerging the explant tissue in a liquid culture medium on a membrane raft, resulting in callus formation. The induction of callus cells by this method provides direct transfer of the cells formed to a liquid medium for a suspension cell culture preparation of taxanes. Thus, there is no need of a separate growth or proliferation step in the method of this invention. Furthermore, callus cells can be produced by the method; and also disclosed is a method for the use of the callus cells so produced in a suspension cell culture preparation for taxanes.
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