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A process for producing a enzymes immobilising carrier, support is made of regenerated porous chitosan in the particle shape and the use of the carrier

机译：一种固定化酶的载体的制备方法，载体由颗粒状的再生多孔壳聚糖制成，并且载体的用途

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摘要

A process for preparing an enzyme-immobilising carrier comprises dissolving a chitosan of low molecular weight in an aqueous acidic solution and adding the solution dropwise to a basic solution to prepare regenerated porous chitosan in particle form, reacting the regenerated porous particulate chitosan with the glycidyl ether of an aliphatic polyalcohol, and subsequently reacting the resulting chitosan with the halide or anhydride of a higher fatty acid in a polar organic solvent. An immobilised lipase is prepared by immobilising the lipase by covalent bonds on the chitosan carrier which is obtained by introducing the glycidyl ether of an aliphatic polyalcohol in a proportion of 0.01 to 0.4 mol to 1 mol of the pyranose ring residue of the chitosan into the chitosan and also by introducing a higher fatty acid with a total number of carbon atoms of from 6 to 20 in a proportion of 0.05 to 1 mol to 1 mol of the pyranose ring residue of the chitosan into the chitosan.

机译：制备固定化酶的载体的方法包括将低分子量的壳聚糖溶解在酸性水溶液中，并将该溶液滴加到碱性溶液中以制备颗粒形式的再生的多孔壳聚糖，使再生的多孔颗粒壳聚糖与缩水甘油醚反应。然后将所得的脱乙酰壳多糖与高级脂肪酸的卤化物或酸酐在极性有机溶剂中反应。通过将脂肪酶通过共价键固定在壳聚糖载体上来制备固定化的脂肪酶，所述壳聚糖载体是通过将脂肪醇多元醇的缩水甘油醚相对于1摩尔的壳聚糖的吡喃糖环残基以0.01至0.4摩尔的比例引入到壳聚糖中而获得的。并且还通过以壳聚糖的吡喃糖环残基的0.05至1摩尔比1摩尔的比例引入碳原子总数为6至20的高级脂肪酸。

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公开/公告号DE4429018C2

专利类型
公开/公告日1996-02-01

原文格式PDF
申请/专利权人 FUJI SPINNING CO. LTD. TOKIO/TOKYO JP;
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申请/专利号DE19944429018
发明设计人 KAWAMURA YOSHIHIDE SHIZUOKA JP;IMAMURA SHIGEYUKI SHIZUOKA JP;HARADA JUNKO SHIZUOKA JP;TANIBE HIROAKI SHIZUOKA JP;
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申请日1994-08-16
分类号C12N11/10;C12N9/34;C12N9/18;C12N9/92;
国家 DE
入库时间 2022-08-22 03:42:16

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