首页>
外国专利>
STRAIN OF BACTERIUM BACILLUS BADIUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5amp;prime;-(A/T)CCGG(A/T)-3amp;prime;
STRAIN OF BACTERIUM BACILLUS BADIUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5amp;prime;-(A/T)CCGG(A/T)-3amp;prime;
FIELD: microbiology, biotechnology. SUBSTANCE: strain of bacterium Bacillus badius ВКМП B-6616 provides preparing restrictase Bba 179 I which recognizes and splits the nucleotide sequence 5′-(A/T)CCGG(A/T)-3′,. Strain does not show demanding to nutrient media, cultivation period is short. Strain B. badius ВКМП B-6616 is isolated from the soil as a result of the search of restrictase producers. The yield of enzyme is 1000 U/g crude biomass, specific activity of the end enzyme is 3000 U/ml. Restrictase Bba 179 I is an isoschizomer of restrictase Bet I. EFFECT: preparing restriction endonuclease indicated above, increased yield of enzyme. 1 dwg
展开▼
机译：领域：微生物学，生物技术。物质：巴德杆菌芽孢杆菌B-6616菌株提供了准备的限制性内切酶Bba 179 I，该酶识别并拆分5'-（A / T）CCGG（A / T）-3'核苷酸序列。菌株对营养培养基的要求不高，栽培周期短。作为限制酶生产者的搜寻结果，从土壤中分离出了B. badius菌株ВКМПB-6616。酶的产量为1000 U / g粗生物质，最终酶的比活为3000 U / ml。限制性酶Bba 179 I是限制性酶Bet I的同分异构体。效果：制备上述限制性内切核酸酶，提高了酶的产量。 1载重吨
展开▼
