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A method for carrying out a laboratory test of the in vitro integrity of haematic cells with no fixation and/or permeabilization, by DNA and general nucleic acid labeling and by flow cytometry reading
A method for carrying out a laboratory test of the in vitro integrity of haematic cells with no fixation and/or permeabilization, by DNA and general nucleic acid labeling and by flow cytometry reading
A method for carrying out a laboratory test of apoptosis of haematic cells in vitro with no fixation/permeabilization procedures, said method comprising the seeding of purified leukocytes, prepared by any way, within a test tube; the dyeing of DNA and of the nucleic acids in general; cold washing in an isotonic solution; suspension in a solution suitable for performing the reading of results by flow cytofluorometry. Reproducible results have been obtained even though the cells, before dyeing the DNA and the nucleic acids in general, are fixed by means of solutions containing typically paraformaldehyde in order to inactivate any pathogens possibly present.
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