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EARLY RECOGNITION PROCESS OF HPV-ASSOCIATED CARCINOMAS OR HIGH-GRADE, HPV-CAUSED DYSPLASIAS

机译：HPV相关性癌或HPV引起的严重异型增生的早期识别过程

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摘要

A process for detecting mRNA that contains HPV-sequences and cellular sequences in an organic sample has the following steps: (a) collecting and preparing an organic sample; (b) isolating mRNA from the organic sample of step (a); (c) translating the thus isolated mRNA into cDNA with a usual primer for reverse transcription; (d) amplifying the thus obtained cDNA by a PCR reaction with a HPV-primer (5' primer) and a primer (3' primer) having sequences of the primer used in step (c); (e) splitting the amplified cDNA obtained in step (d) with an endonuclease that splits the 5' side of the HPV polyadenylating sequence; (f) amplifying the non-split cDNA of step (e) with the primers of step (d) or with nested primers; and (g) detecting the amplifying cDNA of step (f). Also disclosed is the use of such a process for the early recognition of HPV-associated carcinomas or high-grade, HPV-caused dysplasias.
机译：一种检测有机样品中包含HPV序列和细胞序列的mRNA的方法，其步骤如下：（a）收集并制备有机样品; （b）从步骤（a）的有机样品中分离mRNA; （c）用通常的引物将由此分离的mRNA翻译成cDNA以进行逆转录; （d）通过与具有步骤（c）中使用的引物的序列的HPV引物（5'引物）和引物（3'引物）的PCR反应，扩增得到的cDNA; （e）用使HPV聚腺苷酸化序列的5'侧分裂的核酸内切酶分裂在步骤（d）中获得的扩增的cDNA; （f）用步骤（d）的引物或嵌套引物扩增步骤（e）的非分裂cDNA; （g）检测步骤（f）的扩增cDNA。还公开了这种方法用于早期识别HPV相关癌或高度HPV引起的不典型增生的用途。

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