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HETEROGENOUS SPECIFIC BOND TEST USED FOR DETECTION OR CONTENT DETERMINATION OF A GIVEN SUBSTANCE IN A SAMPLE ANALYSED, METHOD OF REDUCING NON-SPECIFIC BONDING IN PSEUDOHOMOGENOUS TESTS AND TESTING SET FOR PERFORMING IMMUNOLOGICAL TEST AND THOSE EMPLOYING GENETIC PROBE
HETEROGENOUS SPECIFIC BOND TEST USED FOR DETECTION OR CONTENT DETERMINATION OF A GIVEN SUBSTANCE IN A SAMPLE ANALYSED, METHOD OF REDUCING NON-SPECIFIC BONDING IN PSEUDOHOMOGENOUS TESTS AND TESTING SET FOR PERFORMING IMMUNOLOGICAL TEST AND THOSE EMPLOYING GENETIC PROBE
A sensitive assay method has been discovered that reduces the amount of non-specific binding present in an assay, the method comprising detecting an analyte present in a sample through a specific binding reaction in which either an analogue of the analyte or a specific binding partner of the analyte is immobilized on a solid-phase and said specific binding reaction produces a detectable product immobilized on said solid- phase that may be correlated to the amount of analyte present in the sample wherein said assay employs an effective amount of a surfactant selected from the group consisting of a polyoxyethylene-alkylether, a polyalkylene oxide-modified polydimethylsiloxane block copolymer, a polyalkylene oxide-modified polymethylsiloxane block copolymer, and mixtures thereof to reduce non-specific binding.
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